Training in Biotechnology in Lucknow| Project in Biotechnology- cytogene.in.
Applications invited for final year Project/Dissertation of B.Sc, M.Sc, B.Tech & M.Tech Students of Life Sciences. For further details please Call us on 8756658021 or 05222355989. Cytogene Research & Development is now Offering Mini- Project of (One and Half Month) 40-45 Days,that will include a complete short term Project.

Biotechnology is the use of living systems and organisms to develop or make useful products, or "any technological application that uses biological systems, living organisms or derivatives thereof, to make or modify products or processes for specific use". Depending on the tools and applications, it often overlaps with the (related) fields of bioengineering and biomedical engineering. For thousands of years, humankind has used biotechnology in agriculture, food production, and medicine. In CytoGene, we offer several modules of training as well as dissertation/project in Biotechnology. The duration of the programs offered for training ranges from 15 &30 days and for project ranges from 3-6 months. Modules are selected by the students on their choice.

TRAINING

ADVANCED BIOTECHNOLOGICAL INTRUMENTATION
Duration- 30 Days ;                                                                                                       Fee- Rs. 85,00/-

Good laboratory practices.
Introduction to microbiology (Basic & Applications)
Demonstration of all Instrument
Chemical calculation & Reagent Preparation

Microbiology:

Media Preparation
Sterilization
Pour Plate Method
Serial Dilution
Spread Plate Method
Spread Plate Method
Streak Plate Method
Culture Broth Preparation
  * Grams staining
  * Acid Fast staining
  * Endospore staining
Casein Hydrolysis Test
Isolation & Morphological study of yeast from food sample

Antibiotic sensitivity test

Molecular Biology

Buffer and stock solution preparation
Determination of Tm value of retrieved DNA sequence
DNA isolation from bacteria
Plasmid DNA isolation & Electrophoresis
PCR Amplification,
DNA isolation from plant leaves & Electrophoresis
RNA isolation from plant leaves
Formaldehyde Gel Electrophoresis
Quantification of genomic DNA &RNA
PCR Amplification & its Electrophoresis
Multiplex PCR & its Electrophoresis

Biochemistry

Isolation of Protein from different sources.
Protein estimation by Lowry’s Method
Separation of amino acids by Thin Layer Chromatography.
Column Chromatography,
Paper Chromatography,
Thin Layer Chromatography
Separation of compounds by Paper Chromatography.
Purification by Column Chromatography.
DNA quantification by UV-Vis Double Beam Spectrophotometer.
RNA quantification by UV-Vis Double Beam Spectrophotometer
Estimation of Sugar by DNS Assay.
Estimation of Cholesterol.

Estimation of Amino Acid

Enzyme & Fermentation Technology

Media Preparation and sterilization.
Screening of enzyme producing bacteria.
Enzyme production from submerged fermentation.
Enzyme production from plant tissue.
Precipitation of enzyme
Estimation by Bradford’s  method
Enzyme Kinetics
Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis.

Recombinant DNA Technology

Sequence retrieval
Primer designing
L.B. Broth preparation & Sterilization
Culturing & multiplication
Plasmid DNA Isolation
Electrophoresis
PCR
Restriction digestion
Ligation and Competent cell preparation
Transformation and Screening of transformants

BASIC BIOTECHNOLOGICAL INSTRUMENDATION

Duration-15 Days ;                                                                                                       Fee- Rs. 5000/-

Good laboratory practices.
Introduction to microbiology (Basic & Applications).
Demonstration of all Instrument.
Chemical calculation & Reagent Preparation.
Media Preparation.
Sterilization.
Testing of Biodiesel.
Pour Plate Method.
Serial Dilution.
Spread Plate Method.
Streak Plate Method.
Culture Broth Preparation.
Grams staining.
Buffer and stock solution preparation.
DNA isolation from bacteria.
Plasmid DNA isolation & Electrophoresis.
DNA isolation from plant leaves & Electrophoresis.
Quantification of genomic DNA.
Isolation of Protein from different sources.
Protein estimation by Lowry’s Method.
Separation of amino acids by Thin Layer Chromatography.
Separation of compounds by Paper Chromatography.
Purification by Column Chromatography.
Estimation of Sugar by DNS Assay.
Enzyme extraction from plants.
Precipitation of enzyme and Quantification of Enzyme

Project/ Dissertation

We offer four different categories of Project/dissertation work depending upon the duration of the project.

Duration: 45 Days; Fee: Rs. 9,000 – 10,000

Duration: 60 Days; Fee: Rs. 12,000- 13,000

Duration: 3 months; Fee: Rs. 15,000 – Rs. 16,000

Duration: 4 months; Fee: Rs. 18,000- Rs. 19,000

Duration 6 months; Fee: Rs. 24,000- Rs, 25,000

A varied range of project/dissertation topics are offered in this division which features major techniques of bacteriology, Mycology, Phycology viz:

Isolation of microbes from a specific source (depending on the topic)  
Preparation of synthetic media  
Pure culture isolation  
Sub-culturing  
Product formulation & production  
Phytochemical Analysis  
Anti-Bacterial Activity test  
Anti-Fungal Activity test  
Biochemical Test  

And Many More..........

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